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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Seviteronel, a Novel CYP17 Lyase Inhibitor and Androgen Receptor Antagonist, Radiosensitizes AR-Positive Triple Negative Breast Cancer Cells
doi: 10.3389/fendo.2020.00035
Figure Lengend Snippet: Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of p-DNAPKcs, total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.
Article Snippet: Proteins were detected using antibodies for phospho-DNAPKcs (Abcam ab124918, 1:1,000),
Techniques: Quantitative RT-PCR, Expressing, Western Blot