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95
Cell Signaling Technology Inc total dnapkcs
Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of <t>p-DNAPKcs,</t> total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.
Total Dnapkcs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Japan SLC inc male immunodeficient (c.b-17/icrhsd-prkdc scid ) mice
Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of <t>p-DNAPKcs,</t> total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.
Male Immunodeficient (C.B 17/Icrhsd Prkdc Scid ) Mice, supplied by Japan SLC inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Santa Cruz Biotechnology anti dna pkcs total
Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of <t>p-DNAPKcs,</t> total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.
Anti Dna Pkcs Total, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Laboratory naïve nod cg prkdc scid il2rg tm1wjl szj nsg mice
Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of <t>p-DNAPKcs,</t> total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.
Naïve Nod Cg Prkdc Scid Il2rg Tm1wjl Szj Nsg Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of p-DNAPKcs, total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.

Journal: Frontiers in Endocrinology

Article Title: Seviteronel, a Novel CYP17 Lyase Inhibitor and Androgen Receptor Antagonist, Radiosensitizes AR-Positive Triple Negative Breast Cancer Cells

doi: 10.3389/fendo.2020.00035

Figure Lengend Snippet: Differential effects on AR and AR targets with enzalutamide and seviteronel treatment. AR+ TNBC cells were treated with 5 μM enzalutamide or seviteronel ± 10 nM DHT. RT-qPCR was used to assess mRNA expression of (A) AR , (B) AQP3 , and (C) SEC14L2 in MDA-MB-453 cells. (D) Protein levels of p-DNAPKcs, total DNAPKcs, and AR were measured by immunoblot in MDA-MB-453 cells. Gene expression data represent mean ± SEM for three independent experiments, and immunoblots are representative of triplicate experiments. NS, p is not significant, * p < 0.05, ** p < 0.01.

Article Snippet: Proteins were detected using antibodies for phospho-DNAPKcs (Abcam ab124918, 1:1,000), total DNAPKcs (CST 12311, 1:1,000), androgen receptor (Millipore 06-680, 1:1,000), GAPDH (CST 2118, 1:1,000), and β-Actin (8H10D10, Cell Signaling 12262S, 1:50,000).

Techniques: Quantitative RT-PCR, Expressing, Western Blot